Ordered catenation of sequence-tagged sites and multiplexed SNP genotyping by sequencing
نویسندگان
چکیده
منابع مشابه
Ordered catenation of sequence-tagged sites and multiplexed SNP genotyping by sequencing.
We describe a method for the efficient genotyping of SNPs, involving sequencing of ordered and catenated sequence-tagged sites (OCS). In OCS, short genomic segments, each containing an SNP, are amplified by PCR using primers that carry specially designed extra nucleotides at their 5'-ends. Amplification products are then combined and converted to a concatamer in a defined order by a second roun...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2002
ISSN: 1362-4962
DOI: 10.1093/nar/30.3.e11